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Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death
Published on: October 31, 2017
Saturated fatty acids alter the late secretory pathway by modulating membrane properties
Laurie-Anne Payet1, Ludovic Pineau, Ellen C R Snyder
1Université de Poitiers, Institut de Physiologie et de Biologie Cellulaires, FRE CNRS 3511, Pôle Biologie-Santé, 1, Rue Georges BONNET, BP 633, 86022, Poitiers Cedex, France.
Traffic (Copenhagen, Denmark)
|September 17, 2013
Summary
Saturated fatty acids cause cell damage by accumulating ceramides and saturated phospholipids. These lipids disrupt the cell
Area of Science:
- Cell Biology
- Lipid Metabolism
- Molecular Biology
Background:
- Saturated fatty acids (SFA) are known to impair organelle function in various cell types.
- SFA accumulation leads to increased ceramides/sphingolipids and saturated phospholipids (PL).
Purpose of the Study:
- To investigate the distinct roles of ceramides and saturated PL in SFA-induced lipotoxicity.
- To elucidate the mechanisms by which these lipids affect the secretory pathway using a yeast model.
Main Methods:
- Utilized a yeast model that mimics mammalian SFA lipotoxicity.
- Analyzed the impact of ceramides and saturated PL on the unfolded protein response, nutrient transporter transcription, and vesicular budding.
Main Results:
- Ceramides modulate the unfolded protein response and nutrient transporter gene expression.
- Saturated PL directly inhibit vesicular budding at the trans-Golgi Network by increasing membrane lipid order.
- This mechanism involves altered recruitment of lipid packing-sensing proteins essential for vesicle formation.
Conclusions:
- Ceramides and saturated PL exert distinct effects on the secretory pathway during SFA lipotoxicity.
- Saturated PL-induced alterations in membrane properties represent a key mechanism impacting the late secretory pathway.
- The findings provide insights into the detrimental effects of fatty acids on cellular secretion.
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