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Related Experiment Videos

c-erbB-2 oncoprotein expression in primary human tumors.

A McCann1, P A Dervan, P A Johnston

  • 1Department of Medical Oncology, Mater Misericordiae Hospital, Dublin.

Cancer
|January 1, 1990
PubMed
Summary

The c-erbB-2 oncoprotein is frequently over-expressed in breast cancer but rarely in other cancers like lung, colon, bladder, prostate, and skin. This protein is easily detectable in standard tissue samples.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Immunohistochemistry

Background:

  • The c-erbB-2 oncoprotein is a transmembrane receptor implicated in various cancers.
  • Understanding its expression patterns across different tumor types is crucial for potential therapeutic targeting.

Purpose of the Study:

  • To investigate the prevalence of c-erbB-2 oncoprotein over-expression in a diverse range of human malignancies.
  • To assess the feasibility of detecting this oncoprotein in routinely processed tissue samples.

Main Methods:

  • Immunohistochemical staining was performed on 405 primary human tumors from breast, lung, colon/rectum, bladder, prostate, and skin.
  • A specific antiserum (21N) was used to detect the c-erbB-2 oncoprotein.

Main Results:

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  • Over-expression was detected in 17% of breast carcinomas, 4% of colorectal tumors, 2% of bladder tumors, and 1% of non-small cell lung carcinomas.
  • No significant over-expression was observed in prostate, skin, or small cell lung carcinomas.
  • The c-erbB-2 oncoprotein was readily identifiable in paraffin-embedded tissues.

Conclusions:

  • The c-erbB-2 oncoprotein over-expression is a common finding in breast cancer.
  • Its over-expression is relatively rare in the other investigated cancer types.
  • Immunohistochemistry provides a reliable method for identifying c-erbB-2 oncoprotein in clinical settings.