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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: May 7, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Assaying multiple restriction endonucleases functionalities and inhibitions on DNA microarray with multifunctional

Lan Ma1, Zhijun Zhu, Tao Li

  • 1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, PR China.

Biosensors & Bioelectronics
|September 17, 2013
PubMed
Summary

A new assay uses gold nanoparticle probes and resonance light scattering to detect restriction endonuclease activity and inhibition. This method offers high sensitivity and shows promise for screening enzyme inhibitors.

Keywords:
DNA microarrayEndonuclease inhibitorGold nanoparticle probeResonance light scatteringRestriction endonuclease

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Last Updated: May 7, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
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Published on: March 7, 2018

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
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Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Area of Science:

  • Biochemistry
  • Nanotechnology
  • Molecular Biology

Background:

  • Restriction endonucleases are crucial enzymes in molecular biology.
  • Assays for detecting enzyme activity and inhibition are vital for research and drug discovery.
  • Existing methods may lack the sensitivity or throughput required for comprehensive screening.

Purpose of the Study:

  • To develop a novel double-stranded (ds) DNA microarray-based assay for studying restriction endonuclease functionality and inhibition.
  • To utilize multifunctional gold nanoparticle (GNP) probes for enhanced detection sensitivity.
  • To demonstrate the assay's capability in simultaneously detecting enzyme activities and quantitatively assessing inhibitor potencies.

Main Methods:

  • A dsDNA microarray platform was employed.
  • Multifunctional gold nanoparticle (GNP) probes were designed and synthesized.
  • Enzyme-cleaved dsDNAs were unwound into single-stranded (ss) DNAs.
  • Hybridization of ssDNAs with complementary ssDNA-functionalized GNP probes.
  • Silver enhancement followed by resonance light scattering (RLS) detection.

Main Results:

  • The assay successfully detected activities of three restriction endonucleases (EcoRI, BamHI, EcoRV) with high specificity.
  • Sensitivity limits were as low as 1.1 × 10(-2) U/mL for BamHI.
  • Quantitative assessment of inhibitory potencies for doxorubicin hydrochloride, ethidium bromide, and an EcoRI-derived helical peptide was achieved.
  • Simultaneous detection of multiple enzyme activities was demonstrated.

Conclusions:

  • A sensitive and specific dsDNA microarray-based RLS assay using GNP probes was successfully developed.
  • The assay is effective for both detecting restriction endonuclease activity and screening inhibitors.
  • This approach holds significant promise for high-throughput screening of endonuclease inhibitors in drug discovery and molecular biology research.