Related Experiment Video
Updated: May 7, 2026

Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste
Published on: July 18, 2025
Biosynthesis of poly(hydroxybutyrate-hydroxyvalerate) from the acclimated activated sludge and microbial
Yuanpeng Wang1, Jiyuan Cai, Jianghuang Lan
1Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, The Key Laboratory for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, PR China.
Abstract:
This study investigated the effects of substrate composition (acetate and propionate) on synthesis of various mix of poly(hydroxybutyrate-hydroxyvalerate) (P(HB/HV)) from activated sludge, which was acclimated using a single carbon (acetate) and mixed carbons (acetate and propionate). Results of batch P(HB/HV) production tests indicated that the yield and synthesis rate of P(HB/HV) decreased as the proportion of propionate in the substrate increased. However, mixed-carbon-acclimated sludge with acetate and propionate exhibited better P(HB/HV) production performance than with acetate-acclimated sludge in terms of substrate utilization, yield of P(HB/HV) and HV fraction in P(HB/HV). The desired hydroxyvalerate (HV) fraction (0-74%) of the P(HB/HV) could be obtained based on the substrate composition and sludge acclimation. Furthermore, PCR-DGGE analysis indicated that specific species dominated the activated sludge used for P(HB/HV) production. Acidobacteria and Burkholderiales were the dominant bacterial populations and played an important role in HV synthesis.
Related Concept Videos
Bioplastics
Production of Organic Acids
Microbial Bioremediation of Plastics
Microbial Bioremediation of Hydrocarbons
Bioremediation

