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Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
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The effects of buffers and pH on the thermal stability, unfolding and substrate binding of RecA

Michael A Metrick1, Joshua E Temple, Gina MacDonald

  • 1Department of Chemistry and Biochemistry, James Madison University, Harrisonburg, VA 22807, United States.

Biophysical Chemistry
|September 17, 2013
PubMed

Keywords:
2-(4-(2-hydroxyethyl)piperazin-1-yl) ethanesulfonic acid2-(N-morpholino) ethanesulfonic acid2′-3′-O-(N-methylanthraniloyl)ATPγSBuffer effectCDCircular dichroismDNA bindingEtBrFRETHEPESMANTMESRecAThermal unfoldingTrisadenosine 5′-O-(thiotriphosphate)circular dichroismdouble-stranded DNAdsDNAethidium bromidefluorescence resonance energy transferpH effectsingle-stranded DNAssDNAtris(hydroxymethyl)aminoethane

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