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Purification of antibodies by precipitating impurities using Polyethylene Glycol to enable a two chromatography step

Glen Giese1, Adam Myrold, Jeffrey Gorrell

  • 1Department of Purification Development, Process Technical Development, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|September 17, 2013
PubMed

Keywords:
AIEXAntibodyCIEXCVDOEDesign of ExperimentsHCPHMWSHPLCHigh Performance Liquid ChromatographyLRVLog Reduction ValueMAbMMVMinute Mouse VirusPEGPolyethylene GlycolPrecipitationPurificationSECanion exchangecation exchangecolumn volumehigh molecular weight specieshost cell proteinmonoclonal antibodysize exclusion chromatography

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