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ACE1 transcription factor produced in Escherichia coli binds multiple regions within yeast metallothionein upstream
C F Evans1, D R Engelke, D J Thiele
1Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor 48109-0606.
Molecular and Cellular Biology
|January 1, 1990
Abstract:
The ACE1 protein of Saccharomyces cerevisiae was expressed as a trpE-ACE1 fusion protein in Escherichia coli and shown to bind CUP1 upstream activation sequences at multiple regions in a copper-inducible manner. These binding sites contain within them the sequence 5'-TC(T)4-6GCTG-3', which we propose constitutes an important part of the ACE1 consensus recognition sequence.