Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Gene Conversion

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

Gene Conversion

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

Continuous Renal Replacement Therapy

Continuous Renal Replacement Therapy

Continuous Renal Replacement Therapy, also known as CRRT, is a procedural treatment for acute kidney injury (AKI) that gradually removes uremic toxins and fluids while maintaining acid-base balance and stabilizing electrolytes. It is particularly useful for hemodynamically unstable patients. Unlike intermittent hemodialysis, which is faster, CRRT provides a gentler approach over 24 hours, closely mimicking the function of natural kidneys. However, CRRT is not ideal for patients with...

Migration

Migration

Migration is long-range, seasonal movement from one region or habitat to another. This common strategy, carried out by many different organisms around the world, is an adaptive response that typically corresponds to changes in an organism’s environment, like resource availability or climate. Migrations can involve huge groups of thousands of animals as well as single individuals traveling alone and can range from thousands of kilometers to just a few hundred meters.

Kidney Transplant I: Introduction

Kidney Transplant I: Introduction

A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...

Spermatogenesis

Spermatogenesis

Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The reconstruction of evolutionary dynamics of processed pseudogenes indicates deep silencing of "retrobiome" in naked mole rat.

Proceedings of the National Academy of Sciences of the United States of America·2024

Same author

Targeted cancer therapy: the initial high concentration may slow down the selection for resistance.

Aging·2024

Same author

From osimertinib to preemptive combinations.

Oncotarget·2024

Same author

My battle with cancer. Part 1.

Oncoscience·2024

Same author

Cancer Relevance of Circulating Antibodies Against LINE-1 Antigens in Humans.

Cancer research communications·2023

Same author

Towards disease-oriented dosing of rapamycin for longevity: does aging exist or only age-related diseases?

Aging·2023

Search research articles

Related Experiment Video

Updated: May 7, 2026

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

Published on: February 1, 2018

S6K in geroconversion.

Olga V Leontieva¹, Zoya N Demidenko, Mikhail V Blagosklonny

¹Department of Cell Stress Biology; Roswell Park Cancer Institute; Buffalo, NY USA.

Cell Cycle (Georgetown, Tex.)

|September 17, 2013

Summary

The mechanistic target of rapamycin (MTOR) pathway influences cellular senescence. Targeting p70S6K, but not MEK, preserves replicative potential during cell cycle arrest, revealing distinct roles in geroconversion.

Area of Science:

Cellular senescence
Molecular biology
Aging research

Background:

Cellular senescence is a key process in aging and disease.
The mechanistic target of rapamycin (MTOR) pathway is implicated in senescence.
Geroconversion describes the transition from reversible to irreversible cell cycle arrest.

Purpose of the Study:

To investigate the distinct roles of p70S6K and MEK in geroconversion.
To determine the impact of p70S6K inhibition on replicative potential during senescence.
To elucidate the relationship between MTOR pathway components and cell cycle arrest.

Main Methods:

Utilized siRNA to inhibit p70S6K expression.
Employed a p70 S6 kinase inhibitor (PF-4708671).

Keywords:

aging cancer cell cycle growth rapalogs senescence

More Related Videos

Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts

Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts

Published on: March 18, 2016

Related Experiment Videos

Last Updated: May 7, 2026

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

Published on: February 1, 2018

Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts

Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts

Published on: March 18, 2016

Assessed replicative potential (RP) and cyclin D1 induction during p21-induced cell cycle arrest.

Main Results:

siRNA for p70S6K partially prevented loss of RP without affecting cyclin D1.
PF-4708671 inhibited S6 phosphorylation and preserved RP, with minimal impact on cyclin D1.
MEK inhibition primarily affects cyclin D1 induction, while p70S6K impacts RP.

Conclusions:

p70S6K and MEK play divergent roles in the geroconversion process.
Targeting p70S6K offers a potential strategy to preserve cellular function during senescence.
Understanding these distinct pathways is crucial for aging and disease research.