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Updated: May 7, 2026
09:17
Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
Published on: August 2, 2018
HINCUTs in cancer: hypoxia-induced noncoding ultraconserved transcripts.
11] Department of Experimental Therapeutics, The Center for RNA Interference and Non-Coding RNAs, MD Anderson Cancer Center, The University of Texas, So Campus Research Building 3, 1881 East Road, Houston, TX 77030, USA [2] Chair of Genetics, Animal Biotechnology and Immunology, Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3 1230, Domzale, Slovenia.
Cell Death and Differentiation
|September 17, 2013
Summary
Hypoxia, or low oxygen, influences long noncoding RNA (lncRNA) expression, including novel hypoxia-induced noncoding ultraconserved transcripts (HINCUTs). One HINCUT supports cancer cell growth in low oxygen, revealing a new regulatory network.
Area of Science:
- Molecular Biology
- Cancer Research
- Genomics
Background:
- Hypoxia is a known feature of the tumor microenvironment.
- MicroRNAs (miRNAs) are linked to hypoxia, but effects on other noncoding transcripts are unknown.
Purpose of the Study:
- To investigate the impact of oxygen deprivation on long noncoding transcripts, specifically transcribed-ultraconserved regions (T-UCRs).
- To identify and characterize hypoxia-modulated T-UCRs and their role in cancer.
Main Methods:
- Genome-wide expression profiling to identify hypoxia-responsive T-UCRs.
- Analysis of clinical colon cancer samples for T-UCR expression.
- Investigating the role of hypoxia-inducible factor (HIF) in T-UCR induction.
- Functional studies of a specific T-UCR (HINCUT-1) in cancer cell proliferation.
Main Results:
- Demonstrated a functional link between oxygen deprivation and T-UCR modulation.
- Identified hypoxia-induced noncoding ultraconserved transcripts (HINCUTs), with several overexpressed in colon cancer.
- Showed HIF is partly responsible for HINCUT induction.
- HINCUT-1 supports cancer cell proliferation under hypoxia and influences protein O-GlcNAcylation.
Conclusions:
- Established a novel functional network linking hypoxia, noncoding RNAs (ncRNAs), and protein-coding transcripts in cancer.
- HINCUTs represent a new class of hypoxia-responsive ncRNAs with potential roles in tumor formation.
- HINCUT-1 is a key player in hypoxic cancer cell adaptation and survival.
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02:39lncRNA - Long Non-coding RNAs
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01:23Hypoxia
Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...