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Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
Published on: May 10, 2022
Type 2 innate lymphoid cells control eosinophil homeostasis.
Jesse C Nussbaum1, Steven J Van Dyken, Jakob von Moltke
1Department of Medicine, University of California San Francisco, San Francisco, California 94143-0795, USA.
Nature
|September 17, 2013
Summary
Long-lived immune cells called type 2 innate lymphoid cells (ILC2) maintain eosinophil levels by releasing interleukin-5. Nutrient intake and circadian rhythms influence this process, impacting eosinophil counts.
Area of Science:
- Immunology
- Cell Biology
Background:
- Eosinophils are key in allergy and parasitic infections.
- Circadian cycling of blood eosinophils is known, but basal regulation is unclear.
- Interleukin-5 (IL-5) and eotaxins are critical for eosinophil function.
Purpose of the Study:
- To elucidate the basal regulation of eosinophil homeostasis.
- To identify the source of constitutive interleukin-5 (IL-5) production.
- To investigate the role of innate lymphoid cells in eosinophil regulation.
Main Methods:
- Characterization of type 2 innate lymphoid cells (ILC2) in tissues.
- Measurement of cytokine (IL-5, IL-13) and chemokine (eotaxins) expression.
- Analysis of ILC2 response to nutrient intake and circadian cues.
Main Results:
- Long-lived tissue-resident ILC2 cells constitutively secrete IL-5.
- ILC2 cells co-express IL-5 and IL-13 in the small intestine, enhanced by caloric intake.
- Vasoactive intestinal peptide stimulates ILC2 cells, linking eosinophils to metabolic cycling.
Conclusions:
- Tissue ILC2 cells are essential for basal eosinophilopoiesis and accumulation.
- ILC2 cytokine expression provides dissociated regulation of eosinophil levels.
- Nutrient intake and circadian rhythms modulate ILC2-driven eosinophil homeostasis.
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