An automated method to prepare cell suspensions from human biopsy samples for immunophenotyping by flow cytometry

Area of Science:

• Biomedical Engineering
• Clinical Laboratory Science
• Hematology

Background:

• Manual preparation of single-cell suspensions from human biopsies is time-consuming.
• Current methods may pose risks of personnel exposure to infectious materials and external contamination.

Purpose of the Study:

• To compare the efficiency and efficacy of an automated versus a manual method for preparing single-cell suspensions from human biopsy samples.
• To evaluate the suitability of the automated method for flow cytometry immunophenotyping and other related assays.

Main Methods:

• An automated instrument utilizing a reciprocating paddle system for fluid shear was compared to a manual method.
• Preparation time, cell viability, and immunophenotyping results were assessed using various human biopsy samples, including B- and T-lymphocytic malignancies.

Main Results:

• The automated method consistently prepared single-cell suspensions significantly faster (15-30 seconds) than the manual method (10-15 minutes).
• Cells isolated via the automated method were viable and suitable for flow cytometry immunophenotyping, yielding comparable results to the manual method.
• The automated technique has been successfully used for over 50 clinical samples.

Conclusions:

• The automated method offers a rapid, efficient, and safe alternative for preparing single-cell suspensions in clinical flow cytometry labs.
• This technology streamlines immunophenotyping and shows potential for other assays involving hematopoietic malignancies.