Coupling biocatalysis with molecular imprinting in a biomimetic sensor

Area of Science:

• Biomimetic sensor technology
• Electropolymer synthesis
• Enzyme catalysis for drug detection

Background:

• Accurate detection of aminopyrine (AP) is vital in pharmaceutical and clinical settings.
• Existing methods for AP detection can be affected by interfering substances like ascorbic acid and uric acid.
• Development of rapid, selective, and sensitive sensors is an ongoing challenge.

Purpose of the Study:

• To develop a novel catalytic biomimetic sensor for the interference-free detection of aminopyrine (AP).
• To achieve sensitive and rapid quantification of AP at submicromolar concentrations.
• To demonstrate the sensor's effectiveness in the presence of common interfering agents.

Main Methods:

• Fabrication of a sensor combining a molecularly imprinted electropolymer with an enzyme.
• Utilizing a peroxide-dependent enzymatic reaction for AP conversion.
• Employing a product-imprinted electropolymer layer on an indicator electrode for signal transduction.
• Testing sensor performance in the presence of ascorbic acid and uric acid.

Main Results:

• The developed sensor achieved interference-free detection of aminopyrine (AP).
• Submicromolar concentrations of AP were detected within a rapid 15-second timeframe.
• The sensor demonstrated high selectivity and sensitivity, even with interfering substances present.

Conclusions:

• The catalytic biomimetic sensor provides a highly effective platform for rapid and selective aminopyrine detection.
• This approach overcomes limitations of conventional methods by eliminating interference.
• The sensor holds significant potential for applications in drug monitoring and quality control.