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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: May 7, 2026

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Published on: March 22, 2020

Intracellular thermometry by using fluorescent gold nanoclusters.

Li Shang¹, Florian Stockmar, Naghmeh Azadfar

¹Institute of Applied Physics and Center for Functional Nanostructures (CFN), Karlsruhe Institute of Technology (KIT), 76131 Karlsruhe (Germany) http://www.aph.kit.edu/nienhaus/

Angewandte Chemie (International Ed. in English)

|September 17, 2013

Summary

Ultrasmall, near-infrared-emitting fluorescent gold nanoclusters (AuNCs) show promise for nanothermometry. These gold nanocluster-based systems offer high thermal sensitivity for simultaneous temperature sensing and imaging in cells.

Keywords:

fluorescence gold nanoclusters imaging sensors temperature

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Area of Science:

Biomedical Engineering
Nanotechnology
Optical Sensing

Background:

Nanothermometry is crucial for understanding cellular processes.
Developing sensitive and biocompatible temperature probes is an ongoing challenge.
Fluorescent nanoparticles offer potential for intracellular thermometry.

Purpose of the Study:

To explore the application of ultrasmall, near-infrared-emitting fluorescent gold nanoclusters (AuNCs) for nanothermometry.
To evaluate the performance of AuNC-based fluorescent nanothermometry in cellular environments.
To assess the potential of AuNCs as a "gold standard" for temperature sensing.

Main Methods:

Synthesis and characterization of ultrasmall, near-infrared-emitting fluorescent gold nanoclusters (AuNCs).
Development of an AuNC-based fluorescent nanothermometry system.
In vitro temperature sensing and imaging experiments using HeLa cells.

Main Results:

AuNC-based fluorescent nanothermometry demonstrated excellent thermal sensitivity.
Simultaneous temperature sensing and imaging were successfully achieved in HeLa cells.
The AuNCs exhibited stable fluorescence and biocompatibility.

Conclusions:

Ultrasmall, near-infrared-emitting fluorescent gold nanoclusters are effective for nanothermometry.
AuNC-based nanothermometry provides a sensitive and simultaneous approach for cellular temperature measurement and imaging.
These findings establish AuNCs as a promising candidate for advanced nanothermometry applications.