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Related Experiment Videos

Lymphocyte subpopulations. Human red blood cell rosettes.

D Tak Yan Yu

    Clinical and Experimental Immunology
    |May 1, 1975
    PubMed
    Summary

    Human red blood cells form rosettes with lymphocytes under specific conditions. These rosettes appear to be a distinct subpopulation of T lymphocytes, showing unique resistance patterns to inhibitory agents.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Human red blood cells (HRBC) can form rosettes with human peripheral blood lymphocytes in vitro.
    • Optimal conditions for HRBC-lymphocyte rosette formation include pH 7.0 and 5% bovine serum albumin (BSA).

    Purpose of the Study:

    • To investigate the characteristics of HRBC rosette-forming cells.
    • To determine if HRBC rosette-forming cells represent a specific lymphocyte subpopulation.

    Main Methods:

    • Studied rosette formation under varying pH and BSA concentrations.
    • Investigated the effect of neuraminidase treatment on rosette formation.
    • Compared HRBC and sheep red blood cell (SRBC) rosette formation after treatment with inhibitory reagents (proteolytic agents, ATS, metabolic inhibitors, cytochalasin B).

    Main Results:

    • HRBC rosette formation is sensitive to pH and BSA concentration.
    • Neuraminidase treatment enhances rosette formation.
    • HRBC rosettes are more resistant to inhibition by proteolytic agents, ATS, metabolic inhibitors, and cytochalasin B compared to SRBC rosettes.
    • Lymphocytes treated with trypsin recovered SRBC rosette-forming ability but lost HRBC rosette-forming ability.

    Conclusions:

    • HRBC rosette-forming cells are likely a subpopulation of thymus-derived (T) lymphocytes.
    • The distinct resistance profile suggests unique surface properties of these cells.

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