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Related Experiment Video

Updated: May 7, 2026

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

An innovative cascade system for simultaneous separation of multiple cell types.

Arkadiusz Pierzchalski¹, Anja Mittag, Jozsef Bocsi

¹Translational Centre for Regenerative Medicine (TRM), University of Leipzig, Leipzig, Germany ; Department of Pediatric Cardiology, Heart Center GmbH, Faculty of Medicine, University of Leipzig, Leipzig, Germany.

|September 17, 2013

Summary

The pluriSelect system offers a faster, simpler method for isolating multiple cell types, including CD4+ and CD8+ T-cells, directly from whole blood compared to traditional magnetic separation techniques.

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Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

Fluorescence-activated cell sorting and magnetic separation are standard methods for isolating cell types but have limitations in cost, time, or effort.
Simultaneous isolation of multiple cell types from a single sample is desirable for efficiency.

Purpose of the Study:

To validate the pluriSelect system for simultaneous isolation of CD4+ and CD8+ T-cells from human EDTA-blood.
To compare the pluriSelect system's performance against magnetic-activated cell sorting (MACS) in terms of purity, yield, and viability.

Main Methods:

The pluriSelect system utilizes antibody-mediated binding to beads of different sizes and isolation via sieves.
Simultaneous separation of CD4+ and CD8+ cells was performed on whole blood using pluriSelect.
Magnetic-activated cell sorting (MACS) was used as a comparative method, involving density gradient isolation of mononuclear cells followed by sequential cell isolation.
Immunophenotyping of isolated and residual cells was conducted using 7-color, 9-marker flow cytometry.

Main Results:

The pluriSelect system demonstrated comparable purity for CD4+ cells but significantly higher purity for CD8+ cells compared to MACS.
No significant differences in yield were observed between the two systems for both CD4+ and CD8+ cells.
Cell viability was slightly higher with MACS for CD4+ cells, but the pluriSelect system showed lower viability for CD8+ cells.
The pluriSelect system was substantially faster (1 hour vs. 2.5 hours) and required no pre-enrichment steps.

Conclusions:

The pluriSelect system is an efficient, fast, and gentle method for simultaneous isolation of multiple cell subpopulations directly from whole blood.
It offers a viable alternative to magnetic separation, particularly for applications requiring rapid, simultaneous isolation of different cell types.