Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Targeting HIV-1 integrase: A review of options, challenges and future directions.

International journal of biological macromolecules·2026

Same author

Immune responses in chicken mucosal lymphoid tissues following oral and eye drop vaccination and revaccination with live modified vaccine of infectious laryngotracheitis virus (ILTV).

Archives of virology·2026

Same author

A rapid test for protein-DNA interactions.

Nucleic acids research·2026

Same author

Rapid screening for acute rheumatic fever using machine learning analysis of host tissue reactive antibodies.

Scientific reports·2025

Same author

Determination of immune responses in mucosal lymphoid tissues following infectious laryngotracheitis virus (ILTV) eye drop and vent brush vaccination of chickens.

Avian pathology : journal of the W.V.P.A·2025

Same author

Delayed Surgery and Adenosine, Lidocaine, and Mg<sup>2+</sup> Immunomodulatory Therapy Improve Joint Recovery in a Sex-Specific Manner After Anterior Cruciate Ligament Reconstruction in a Rat Model.

The American journal of sports medicine·2025

Search research articles

Related Experiment Video

Updated: May 7, 2026

DNA-barcode-based Multiplex Immunofluorescence Imaging to Analyze FFPE Specimens from Genetically Reprogrammed Murine Melanoma

DNA-barcode-based Multiplex Immunofluorescence Imaging to Analyze FFPE Specimens from Genetically Reprogrammed Murine Melanoma

Published on: June 6, 2025

Improved diagnosis of melioidosis using a 2-dimensional immunoarray.

Alanna E Sorenson¹, Natasha L Williams, Jodie L Morris

¹School of Pharmacy and Molecular Sciences, James Cook University, Douglas QLD 4811, Australia.

Diagnostic Microbiology and Infectious Disease

|September 18, 2013

Summary

New serological assays, ELISA and 2D immunoarray, offer faster and more reliable diagnosis for melioidosis, caused by Burkholderia pseudomallei. These tests improve detection rates in patients missed by current methods, aiding timely treatment.

Keywords:

Burkholderia pseudomallei Diagnostics ELISA Melioidosis Serology Two-dimensional Immunoarray

More Related Videos

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

Published on: June 15, 2018

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Related Experiment Videos

Last Updated: May 7, 2026

DNA-barcode-based Multiplex Immunofluorescence Imaging to Analyze FFPE Specimens from Genetically Reprogrammed Murine Melanoma

DNA-barcode-based Multiplex Immunofluorescence Imaging to Analyze FFPE Specimens from Genetically Reprogrammed Murine Melanoma

Published on: June 6, 2025

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

Published on: June 15, 2018

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Area of Science:

Microbiology
Immunology
Infectious Diseases

Background:

Melioidosis, caused by Burkholderia pseudomallei, presents diagnostic challenges with current gold standard culture methods requiring days for results.
Existing serological tests like the indirect hemagglutination assay (IHA) have limitations, including missed diagnoses and persistently seronegative patients.

Purpose of the Study:

To develop and evaluate novel serological assays for improved melioidosis diagnosis.
To enhance the detection of antibodies in patient sera, particularly in cases missed by conventional methods.

Main Methods:

Development of an enzyme-linked immunosorbent assay (ELISA) and a 2-dimensional immunoarray (2DIA).
Evaluation of assay performance using patient sera, comparing sensitivity and specificity against culture and existing IHA.

Main Results:

The 2DIA demonstrated 100% sensitivity and 87.1% specificity, detecting all culture-positive, IHA-negative samples.
The ELISA showed 86.2% sensitivity and 93.5% specificity, detecting 67% of culture-positive, IHA-negative samples.
Both ELISA and 2DIA detected antibodies in a greater proportion of IHA-negative patient subsets compared to IHA.

Conclusions:

ELISA and 2DIA represent rapid and reliable alternatives for melioidosis serological testing.
These new assays improve diagnostic capabilities, especially for challenging cases missed by the IHA.
Enhanced serological testing can facilitate earlier and more successful treatment of acute melioidosis.