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Updated: May 7, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

Electrical percolation based biosensors.

Hugh Alan Bruck¹, Minghui Yang, Yordan Kostov

¹University of Maryland College Park (UMCP), College Park, MD 20742, United States.

Methods (San Diego, Calif.)

|September 18, 2013

Summary

This summary is machine-generated.

A novel label-free biosensor utilizes electrical percolation in carbon nanotube networks to detect biological molecules. This technology enables real-time, electronic measurement of antigen-antibody interactions with high sensitivity.

Keywords:

Antibody Biosensor Carbon nanotubes Electrical percolation Semiconductor

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Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor

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Published on: February 10, 2014

Area of Science:

Biomedical Engineering
Nanotechnology
Biosensing

Background:

Label-free biosensing is crucial for direct detection of biological molecules.
Electrical percolation offers a novel physical principle for electronic sensing.
Carbon nanotube networks provide a versatile platform for biosensor development.

Purpose of the Study:

To develop a label-free biosensor based on electrical percolation.
To demonstrate the electronic detection of biological interactions using a biological semiconductor (BSC).
To enable real-time, continuous monitoring of analytes.

Main Methods:

Fabrication of a biological semiconductor (BSC) using a 3-D carbon nanotube-antibody network on a PMMA substrate.
Utilizing the change in electrical resistance due to antigen-antibody binding disrupting network continuity.
Demonstrating detection of staphylococcal enterotoxin B (SEB) using anti-SEB IgG functionalized SWNTs.

Main Results:

The BSC demonstrated label-free electronic detection of SEB at concentrations as low as 1 ng/ml.
Antigen binding significantly increased the electrical resistance of the SWNT network.
An automated configuration enabled real-time continuous detection.

Conclusions:

Electrical percolation is a viable principle for label-free electronic biosensing.
The developed BSC offers a sensitive and direct method for analyte detection.
Future applications include multi-analyte detection arrays and "biological CPUs".