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Updated: May 7, 2026
Generation of Recombinant Influenza Virus from Plasmid DNA
Published on: August 3, 2010
[Reverse genetics platform construction of influenza pandemic virus strain]
Hong Bo1, Ye Zhang, Wei-Juan Huang
1National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Objective:
Reverse genetics was used to construct the platform of flu pandemic strain A/California/07/2009 (H1N1).
Methods:
Eight genes fragements were amplified and ligated with bidirectional vector, recombinant plasmids were co transfected to the 293 T cells and rescued the virus. Gene sequencing, antigenic analysis and growth property were used to evaluate the rescued virus.
Results:
Rescued virus show the genes sequence correct, keep the same antigenicity and similar growth property compared with wild type virus.
Conclusion:
The pandemic virus reverse genetics platform of A/California/07/2009 (H1N1) were built. Based on this platform, rescued virus hold the similarity of antigenicity and growth ability with wild type virus.
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