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Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System
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[Establishment of a purificatory method for alpha-fetoprotein variant by affinity adsorption]
Yong-Ji Song1, Jun Hou, Jun Xu
1Center of Clinical Laboratory, No. 302 Hospital of the PLA, Beijing 100039, China.
Objective:
To establish a purificatory method of alpha-fetoprotein variant (AFP-L3) based on microspincolumn with lens culinaris agglutinin (LCA).
Methods:
LCA was isolated by ammonium sulfate precipitation method from lens culinaris. AFP-L3 affinity adsorption microspincolumns which were made from LCA coupled with activated Sepharose 4B were prepared. By adding into the centrifuge column, serum was absorbed and eluted to purify AFP-L3. The results of purified AFP-L3 detection of 10 cases AFP positive sera by electro-chemiluminescence immunoassay were compared with traditional crossed affinity immunoelectrophoresis.
Results:
8 of 10 cases AFP-L3 concentration were greater than 5 ng/ml in purified sera. Six cases show positive reaction in affinity immune cross electrophoresis experiment.
Conclusion:
Successfully established purification method of AFP-L3 by affinity absorption based on microspincolumn. The method was more conducive to clinical laboratory applications due to its high sensitive and easy operation.
