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Related Concept Videos

Lymphatic Vessels and Lymph Transport01:16

Lymphatic Vessels and Lymph Transport

Lymphatic vessels, known as lymphatics, are crucial in transporting lymph from peripheral tissues to our venous system. This process begins with lymph entering through tiny capillaries that branch through tissues. These capillaries have unique features such as larger diameters, thinner walls, and a distinctive one-way valve system formed by overlapping endothelial cells.
This one-way system allows fluids, solutes, and even pathogens to enter but prevents their return to the intercellular spaces.
Detailed Structure and Function of Lymph Nodes01:23

Detailed Structure and Function of Lymph Nodes

Lymph nodes are bean-shaped structures that cluster along the lymphatic vessels in the inguinal, axillary, and cervical regions. Each node is divided into compartments by a capsule that extends trabeculae inward.
From a histological perspective, lymph nodes can be split into two main areas: the superficial cortex and the deep medulla. The outer cortex is populated by dendritic cells, macrophages, and B lymphocytes, which are densely packed into follicles. When these B-lymphocytes are presented...
Functions of the Lymphatic and Immune System01:28

Functions of the Lymphatic and Immune System

The lymphatic system plays a crucial role in bolstering our immune system. It consists of a network of lymphoid organs, lymph, and lymphatic vessels that provide structural and functional support in safeguarding the body against pathogens such as viruses and bacteria.
The primary lymphoid organs, including the bone marrow and the thymus, serve as the maturation sites for lymphocytes. Secondary lymphoid organs, like the mucosa-associated lymphoid tissue, activate these lymphocytes and serve as...
Veins of Head and Neck01:19

Veins of Head and Neck

The blood drainage from the head and neck is primarily managed by three pairs of veins: the external jugular, internal jugular, and vertebral veins. The external jugular veins drain superficial scalp and face structures, passing over the sternocleidomastoid muscles to empty into the subclavian veins.
On the other hand, the vertebral veins, unlike their arterial counterparts, are not primarily responsible for brain drainage. Instead, they drain the cervical vertebrae, spinal cord, and some small...
Secondary Lymphoid Organs01:15

Secondary Lymphoid Organs

Secondary organs, including lymph nodes, the spleen, and mucosa-associated lymphoid tissue (MALT), work harmoniously to protect us from disease and infection.
The spleen is a vital organ in the lymphatic system, nestled in the upper left side of the abdomen. It is composed of two primary regions: the red pulp and the white pulp, each having distinct functions. The red pulp performs a significant role in blood filtration. It efficiently purges the blood of old or damaged red blood cells and...
Introduction to Lymphatic and Immune System01:23

Introduction to Lymphatic and Immune System

Immunity is a crucial biological concept about our body's inherent capacity to prevent infections and diseases. A complex network of cells and tissues collectively known as the immune system facilitates this natural defense mechanism. The immune system plays an integral role in maintaining our health and well-being, shielding us from potential health threats.
The immune responses can be categorized into two types: innate and adaptive. Innate immunity comprises nonspecific defenses we are born...

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Related Experiment Video

Updated: May 7, 2026

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice
05:59

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

Published on: May 14, 2020

Lymphatic vessel function in head and neck inflammation.

Lucy A Truman1, Noelia A-Gonzalez, Kevin L Bentley

  • 11 Department of Epidemiology of Microbial Diseases, Yale School of Public Health, Yale University School of Medicine , New Haven, Connecticut.

Lymphatic Research and Biology
|September 19, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed ProxTom mice to visualize lymphatic vessels in living animals. This tool aids in studying lymphangiogenesis and isolating lymphatic endothelial cells during immune responses.

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Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy
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Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy

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Last Updated: May 7, 2026

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice
05:59

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

Published on: May 14, 2020

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy
10:05

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy

Published on: May 22, 2020

Area of Science:

  • Immunology
  • Vascular Biology
  • Molecular Imaging

Background:

  • Head and neck infections can cause lymphedema, leading to airway obstruction.
  • Lymphangiogenesis, the formation of new lymphatic vessels, occurs during head and neck infections and after immunization.
  • Studying lymphatic endothelial cells is crucial for understanding inflammatory responses.

Purpose of the Study:

  • To develop tools for in vivo imaging of lymphatic vessels.
  • To enable isolation of individual lymphatic endothelial cells for quantitative analysis.
  • To investigate changes in lymphatic vessels during inflammation.

Main Methods:

  • Developed the ProxTom transgenic mouse with a red-fluorescent reporter (td-Tomato) under Prox1 regulatory control.
  • Imaged lymphatic vessels in lymph nodes of immunized mice in vivo.
  • Isolated fluorescent lymphatic endothelial cells based on td-Tomato expression.

Main Results:

  • The ProxTom transgene accurately reflects endogenous Prox1 expression in lymphatic vessels.
  • Demonstrated in vivo lymphangiogenesis following immunization.
  • Established a method for isolating lymphatic endothelial cells using td-Tomato fluorescence.

Conclusions:

  • ProxTom mice are valuable tools for in vivo studies of lymphatic vessels during immune responses.
  • The td-Tomato reporter faithfully marks lymphatic vessels, facilitating research.
  • ProxTom mice are available for distribution, promoting further research in lymphatic biology.