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'Switch-off' biosensing for chymotrypsin-catalyzed reaction by SPR-SERS spectroscopy
Cuicui Fu1, Weiqing Xu, Gang Chen
1State Key Laboratory of Supramolecular Structure and Materials, Jilin University, 2699 Qianjin Ave., Changchun 130012, P. R. China. xusp@jlu.edu.cn.
The Analyst
|September 19, 2013
Abstract:
We describe a novel 'switch-off' biosensing strategy for the detection of chymotrypsin based on surface plasmon resonance (SPR) and surface-enhanced Raman scattering (SERS) spectroscopy. This approach analyzes the fingerprint spectrum of the chymotrypsin-catalyzed substrate. The lowest probed concentration is 0.4 nM for chymotrypsin.
