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Updated: May 7, 2026

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

Processing of projections containing phase contrast in laboratory micro-computerized tomography imaging.

Zdenko Zápražný¹, Dušan Korytár, Petr Mikulík

¹Institute of Electrical Engineering, Slovak Academy of Sciences, Dúbravská cesta 9, SK-841 04 Bratislava, Slovakia.

Journal of Applied Crystallography

|September 19, 2013

Summary

This study demonstrates phase contrast imaging with laboratory X-ray sources. Phase retrieval methods enhance X-ray projections of lightweight objects, improving spatial resolution.

Keywords:

X-ray imaging X-ray radiography computed radiography computerized tomography digital radiography phase-contrast imaging

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High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

Published on: June 21, 2011

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Published on: October 24, 2019

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

Published on: June 21, 2011

Area of Science:

Physics
Materials Science
Imaging Technology

Background:

Free-space-propagation-based imaging uses X-ray phase contrast.
High spatial coherence is essential for X-ray phase contrast imaging.
Laboratory microfocus sources enable synchrotron-based techniques.

Purpose of the Study:

To test phase retrieval methods for X-ray images in laboratory settings.
To evaluate the processing of phase contrast X-ray images of lightweight objects.
To compare simulated and recorded X-ray projection results.

Main Methods:

Utilizing a laboratory imaging system with a microfocus X-ray source.
Employing a high-resolution CCD camera for data acquisition.
Processing simulated and recorded X-ray projections using phase retrieval algorithms.

Main Results:

Phase contrast imaging was successfully applied to lightweight objects.
Phase retrieval methods improved edge enhancement in X-ray projections.
Qualitative comparison showed the effectiveness of the laboratory approach.

Conclusions:

Laboratory-based phase contrast X-ray imaging is feasible.
Phase retrieval techniques can enhance spatial resolution and reduce artifacts.
This approach offers a viable alternative to synchrotron-based methods.