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Updated: May 7, 2026

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
Published on: October 20, 2014
MBNL1 and RBFOX2 cooperate to establish a splicing programme involved in pluripotent stem cell differentiation
Julian P Venables1, Laure Lapasset, Gilles Gadea
11] CNRS, Institut de Génétique Moléculaire de Montpellier, Montpellier F-34293, France [2] Institute of Genetic Medicine, Newcastle University, Newcastle-upon-Tyne NE1 3BZ, UK [3] Université Montpellier 2, F-34293 Montpellier cedex 5, France [4] Université Montpellier 1, F-34094 Montpellier cedex 5, France [5].
Abstract:
Reprogramming somatic cells into induced pluripotent stem cells (iPSCs) has provided huge insight into the pathways, mechanisms and transcription factors that control differentiation. Here we use high-throughput RT-PCR technology to take a snapshot of splicing changes in the full spectrum of high- and low-expressed genes during induction of fibroblasts, from several donors, into iPSCs and their subsequent redifferentiation. We uncover a programme of concerted alternative splicing changes involved in late mesoderm differentiation and controlled by key splicing regulators MBNL1 and RBFOX2. These critical splicing adjustments arise early in vertebrate evolution and remain fixed in at least 10 genes (including PLOD2, CLSTN1, ATP2A1, PALM, ITGA6, KIF13A, FMNL3, PPIP5K1, MARK2 and FNIP1), implying that vertebrates require alternative splicing to fully implement the instructions of transcriptional control networks.
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