Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification and initial characterisation of Trypanosoma Cox17 copper metallochaperone.

Molecular and biochemical parasitology·2025
Same author

Expression and copper binding studies of a Plasmodium falciparum protein with Cox19 copper binding motifs.

Experimental parasitology·2023
Same author

Expression and copper binding characteristics of Plasmodium falciparum cytochrome c oxidase assembly factor 11, Cox11.

Malaria journal·2022
Same author

Isolation of Nile crocodile (Crocodylus niloticus) serum immunoglobulin M and Y (IgM and IgY).

Journal of immunological methods·2019
Same author

Expression and copper binding properties of the N-terminal domain of copper P-type ATPases of African trypanosomes.

Molecular and biochemical parasitology·2019
Same author

Monocyte phagocytosis of malaria β-haematin in the presence of artemisinin, amodiaquine, chloroquine, doxycycline, primaquine, pyrimethamine and quinine.

Experimental parasitology·2018

Related Experiment Video

Updated: May 7, 2026

Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis
10:50

Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis

Published on: November 2, 2018

Malaria rapid diagnostic tests: challenges and prospects.

Joel C Mouatcho1, J P Dean Goldring1

  • 1Department of Biochemistry, School of Life Science, University of Kwazulu-Natal, Pietermaritzburg, Private Bag X01 Scottsville 3209, South Africa.

Journal of Medical Microbiology
|September 20, 2013
PubMed
Summary
This summary is machine-generated.

Malaria rapid diagnostic tests (RDTs) face challenges with accuracy and reliability due to antigen variability. New RDTs are exploring alternative targets to improve detection of Plasmodium species.

More Related Videos

A Multi-detection Assay for Malaria Transmitting Mosquitoes
09:00

A Multi-detection Assay for Malaria Transmitting Mosquitoes

Published on: February 28, 2015

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Related Experiment Videos

Last Updated: May 7, 2026

Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis
10:50

Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis

Published on: November 2, 2018

A Multi-detection Assay for Malaria Transmitting Mosquitoes
09:00

A Multi-detection Assay for Malaria Transmitting Mosquitoes

Published on: February 28, 2015

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Area of Science:

  • * Tropical Medicine
  • * Parasitology
  • * Biotechnology

Background:

  • * Growing interest in malaria rapid diagnostic tests (RDTs) for detecting Plasmodium species.
  • * Current RDTs primarily target Plasmodium falciparum histidine-rich protein 2 (PfHRP2), plasmodial aldolase, and plasmodial lactate dehydrogenase (pLDH).
  • * Existing RDTs exhibit significant variability in sensitivity, specificity, and temperature tolerance, posing diagnostic challenges.

Purpose of the Study:

  • * To review recent advancements in malaria RDTs.
  • * To discuss the limitations of current RDTs targeting PfHRP2, pLDH, and aldolase.
  • * To explore novel antigen targets for next-generation malaria RDTs.

Main Methods:

  • * Comprehensive literature review of malaria RDTs.
  • * Analysis of RDT performance data for PfHRP2, pLDH, and aldolase targets.
  • * Discussion of challenges including genetic variability and antigen persistence.

Main Results:

  • * PfHRP2-based RDTs dominate the market but face issues due to genetic diversity of the Pfhrp2 gene.
  • * Variability in test performance (sensitivity, specificity, false positives/negatives) is a significant concern.
  • * Antigen persistence in blood after parasite clearance can lead to false positive results.

Conclusions:

  • * Current malaria RDTs face substantial challenges impacting their diagnostic accuracy.
  • * Genetic variability in target antigens and antigen persistence are key limitations.
  • * Development of RDTs with alternative antigen targets is crucial for improved malaria diagnosis.