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Updated: May 7, 2026
12:37
Pyrosequencing for Microbial Identification and Characterization
Published on: August 22, 2013
Detection of heteroresistant Mycobacterium tuberculosis by pyrosequencing
Anna Engström1, Sven Hoffner, Pontus Juréen
1Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
Journal of Clinical Microbiology
|September 20, 2013
Abstract:
The ability of pyrosequencing to detect a resistant minority population of a heteroresistant Mycobacterium tuberculosis strain was investigated by performing a titration study. A mutant signal was noted only when the proportion of mutant DNA in the DNA target was 35 to 50%, showing that the sensitivity is significantly lower than that of phenotypic drug susceptibility test methods.
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