Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A microplaque reduction assay for human and mouse interferon.

J B Campbell, T Grunberger, M A Kochman

    Canadian Journal of Microbiology
    |August 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A "Refuse" Case.

    The Homoeopathic physician·2023
    Same author

    Phosphorus-A Peculiar Symptom.

    The Homoeopathic physician·2023
    Same author

    Screening and diagnosis of gestational diabetes.

    BMJ (Clinical research ed.)·2023
    Same author

    Dragon 1 Protocol Manuscript: Training, Accreditation, Implementation and Safety Evaluation of Portal and Hepatic Vein Embolization (PVE/HVE) to Accelerate Future Liver Remnant (FLR) Hypertrophy.

    Cardiovascular and interventional radiology·2022
    Same author

    DNA methylation signatures in cord blood associated with birthweight are enriched for dmCpGs previously associated with maternal hypertension or pre-eclampsia, smoking and folic acid intake.

    Epigenetics·2021
    Same author

    The estimated effect of reducing the maternal smoking rate on neonatal intensive care unit costs in Victorian public hospitals.

    Australian health review : a publication of the Australian Hospital Association·2021

    A new microplaque reduction assay for human and mouse interferons is presented. This rapid, reproducible method using disposable plates is suitable for automation and other virology applications.

    Area of Science:

    • Immunology
    • Virology
    • Assay Development

    Background:

    • Interferons (IFNs) are crucial cytokines in antiviral immunity.
    • Accurate and efficient assays are needed to quantify IFN activity.
    • Existing methods may be time-consuming or lack automation potential.

    Purpose of the Study:

    • To describe a novel microplaque reduction assay for human and mouse interferons.
    • To evaluate the assay's speed, reproducibility, and automation potential.
    • To discuss broader applications in virology.

    Main Methods:

    • Utilized disposable, multicompartmental plastic plates with 6 mm flat-bottomed wells.
    • Developed a microplaque reduction assay protocol for interferon quantification.
    • Assessed assay performance for human and mouse interferon samples.

    Related Experiment Videos

    Main Results:

    • The assay is rapid and reproducible.
    • The microplaque format is amenable to automation.
    • Demonstrated successful application for interferon measurement.

    Conclusions:

    • The described microplaque reduction assay offers an efficient method for quantifying interferons.
    • The assay has potential for automation and broader use in virology, including virus screening and antibody assays.