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Related Experiment Video

Updated: May 7, 2026

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

An introduction to fluorescence imaging techniques geared towards biosensor applications.

J Goedhart¹, Mark A Hink, Kees Jalink

¹Section of Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)

|September 21, 2013

Summary

This chapter details fluorescence and Förster Resonance Energy Transfer (FRET) recording methods, focusing on microscopy for biosensor imaging. It guides instrument selection, detection techniques, and practical considerations for successful FRET experiments.

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FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

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Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

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Published on: November 2, 2009

Area of Science:

Biophysics
Molecular Biology
Microscopy

Background:

Fluorescence and Förster Resonance Energy Transfer (FRET) are fundamental biophysical techniques.
FRET is widely utilized in biosensor imaging for studying molecular interactions.
Effective FRET recording is crucial for accurate biological interpretation.

Purpose of the Study:

To provide a comprehensive overview of FRET recording methodologies.
To emphasize microscopy-based techniques for biosensor applications.
To offer practical guidelines for experimental design and execution.

Main Methods:

Discussion of basic fluorescence and FRET principles.
Detailed examination of microscopy methods for FRET detection.
Exploration of intensity-based and donor lifetime-based FRET measurements.

Main Results:

Guidance on selecting appropriate instruments for FRET recording.
Strategies for matching recording methods to specific experimental needs.
Identification of critical practical considerations impacting experimental success.

Conclusions:

Microscopy offers versatile FRET detection for biosensor imaging.
Careful instrument selection and method matching enhance FRET experiment reliability.
Addressing practical challenges is key to successful FRET data acquisition.