HN(CA)N and HN(COCA)N experiments for assignment of large disordered proteins

Two new 3D HN-based experiments are proposed for backbone assignment of large disordered proteins. The spectra obtained with the new pulse schemes are free of redundant diagonal peaks (HiNi-Ni) and provide sequential correlations (HiNi-Ni+1 and HiNi-Ni-1) not only between adjacent non-proline residues but also between non-proline and proline residues. The experiments have been demonstrated on an intrinsically disordered protein with 306 amino acids including 64 proline residues. Using the two experiments, we obtained nearly complete assignments of backbone amides and proline (15)N spins except for 4 proline and 4 non-proline residues.