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Updated: May 7, 2026
09:40
Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
Published on: October 4, 2019
Identification of aberrant microRNA expression pattern in pediatric gliomas by microarray
Fatao Liu1, Yuyu Xiong, Yang Zhao
1Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 294 Taiyuan Road, Shanghai 200031, PR China. majie365@163.com.
Diagnostic Pathology
|September 24, 2013
Summary
This study identified 40 microRNAs with altered expression in pediatric gliomas, including novel cancer-related microRNAs. These findings enhance understanding of pediatric brain tumor biology and may aid in developing new therapies and diagnostic markers.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Pediatric brain tumors are a leading cause of childhood cancer death.
- The role of microRNAs in pediatric brain tumor genesis is largely unknown.
Purpose of the Study:
- To investigate the expression patterns of microRNAs in pediatric gliomas.
- To identify novel microRNAs involved in the development of pediatric brain tumors.
Main Methods:
- MicroRNA microarray assay was used to analyze microRNA expression in pediatric gliomas and matched normal tissues.
- Quantitative reverse transcription PCR (qRT-PCR) was employed to validate the expression of selected microRNAs.
Main Results:
- Forty differentially expressed microRNAs were identified in pediatric gliomas.
- miR-1321, miR-513b, and miR-769-3p were found to be associated with cancer genesis.
- Gene Ontology (GO) and pathway analysis revealed enrichment of target genes in nervous system and tumor-related biological processes.
Conclusions:
- This study identified altered microRNA expression patterns in pediatric gliomas.
- The findings contribute to understanding pediatric brain tumor biology.
- This research may support the development of targeted therapies and improved disease stratification markers.
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...