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Updated: May 7, 2026

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
Published on: June 6, 2025
Optimization of antioxidant exopolysaccharidess production by Bacillus licheniformis in solid state fermentation
Yaowei Fang1, Sibtain Ahmed, Shu Liu
1School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang, Jiangsu Province 222005, China; University of California San Diego, 9500 Gilman Drive La Jolla, CA 92093, USA.
Abstract:
Response surface methodology was applied to optimize physical and nutritional variables for the production of antioxidant exopolysaccharidess (EPSs) by Bacillus licheniformis UD061 in solid state fermentation with squid processing byproduct and maize cob meal used as a carbon and nitrogen source and solid matrix. The factors noted with Plackett-Burman design for optimization of EPSs production were NaCl, MgSO4·7H2O, and moisture level. These factors were further optimized using Box-Behnken design and response surface methodology. Using this methodology, the quadratic regression model of EPSs production was built. Maximum EPSs production was obtained under the optimal conditions of 4.08 g L(-1) NaCl, 0.71 g L(-1) MgSO4·7H2O, and 60.49% moisture level. A production of 14.68 mg gds(-1), which was well in agreement with the predicted value, was achieved by this optimized procedure.
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