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A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures
Published on: February 10, 2011
PNA-FISH assays for early targeted bacteraemia treatment
1Department of Medical Microbiology, Ninewells Hospital and Medical School, NHS Tayside, Dundee, United Kingdom.
Journal of Microbiological Methods
|September 24, 2013
Abstract:
PNA-FISH S. aureus/CNS and GNR Traffic Light assays were compared with standard culture methods for identifying bacteraemia in 156 blood cultures from 131 patients. Results correlated with final culture results in 153 cultures. Retrospective case note review revealed that earlier targeted treatment would have occurred in 10.7% of cases.
Keywords:
APIAnalytical profile indexBacteraemiaBlood culturesCLSIClinical and Laboratory Standards InstituteGNRGPCGram negative rodsGram positive cocciMALDI-TOF MSMRSAMSSAMatrix-assisted laser desorption/ionisation-time of flight mass spectrometryMeticillin-resistant Staphylococcus aureusMeticillin-sensitive Staphylococcus aureusPCRPNA-FISHPeptide nucleic acid fluorescence in situ hybridizationPolymerase chain reactionTreatment
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