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Published on: March 30, 2019

miR-370 targeted FoxM1 functions as a tumor suppressor in laryngeal squamous cell carcinoma (LSCC)

Wu Yungang1, Li Xiaoyu2, Taizhong Pang2

  • 1Department of Otolaryngology-Head and Neck Surgery, Qi-Lu Hospital, Shandong University, Jinan, Shandong Province, PR China; Department of Otolaryngology-Head and Neck Surgery, the Affiliated Hospital of Jining Medical University, Jining, Shandong Province, PR China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|September 24, 2013
PubMed

Insights

MicroRNA-370 (miR-370) is down-regulated in laryngeal squamous cell carcinoma (LSCC). Restoring miR-370 inhibits LSCC cell proliferation by targeting Forkhead Box M1 (FoxM1), suggesting its tumor suppressor role.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs regulate gene expression and are implicated in various cancers.
  • Laryngeal squamous cell carcinoma (LSCC) pathogenesis involves aberrant microRNA expression.
  • The specific role of miR-370 in LSCC has not been fully elucidated.

Purpose of the Study:

  • To investigate the expression and function of miR-370 in laryngeal squamous cell carcinoma.
  • To identify potential targets of miR-370 within LSCC.
  • To evaluate the therapeutic potential of miR-370 in LSCC.

Main Methods:

  • Quantitative analysis of miR-370 expression in LSCC tissues.
  • Bioinformatic prediction and luciferase reporter assays to validate miR-370 targets.
  • Cell proliferation assays in LSCC cell lines (Hep2) following miR-370 restoration.

Main Results:

  • miR-370 expression was significantly down-regulated in human LSCC tissues.
  • An inverse correlation was observed between miR-370 and Forkhead Box M1 (FoxM1) expression in LSCC.
  • FoxM1 was confirmed as a direct target of miR-370.
  • Restoration of miR-370 suppressed proliferation in Hep2 LSCC cells.

Conclusions:

  • miR-370 acts as a tumor suppressor in LSCC by down-regulating FoxM1.
  • miR-370 holds potential as a diagnostic marker and therapeutic target for LSCC.
Keywords:
Cell proliferationFoxM1Laryngeal squamous cell carcinomaMiR-370

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