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Related Experiment Video

Updated: May 7, 2026

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

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Published on: August 16, 2016

Microfluidic passive permeability assay using nanoliter droplet interface lipid bilayers.

Takasi Nisisako1, Shiva A Portonovo, Jacob J Schmidt

  • 1Department of Bioengineering, University of California, Los Angeles, CA 90095-1600, USA. schmidt@seas.ucla.edu nisisako.t.aa@m.titech.ac.jp.

The Analyst
|September 24, 2013
PubMed
Summary

This study introduces a novel microfluidic assay using droplet interface bilayers (DIBs) to more accurately measure drug permeability across artificial membranes. The new method offers a faster and more reliable approach for drug discovery screening.

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Area of Science:

  • Biophysical Chemistry
  • Microfluidics
  • Drug Discovery

Background:

  • Conventional artificial membrane permeability assays (PAMPA) lack physical and chemical similarity to biological membranes.
  • Accurate membrane permeability assessment is crucial for evaluating drug transport.

Purpose of the Study:

  • To develop a microfluidic passive permeability assay utilizing droplet interface bilayers (DIBs).
  • To improve the accuracy and efficiency of drug permeability assays.

Main Methods:

  • Formation of nanoliter-sized aqueous droplets in a microfluidic network with phospholipids.
  • Creation of planar droplet interface bilayers (DIBs) by oil removal.
  • Measurement of fluorescein and caffeine permeation using fluorometry and UV microspectroscopy.

Main Results:

  • Calculated effective permeabilities for fluorescein at different pH values (5.1–87.6 × 10⁻⁶ cm s⁻¹).
  • Determined intrinsic permeabilities for specific fluorescein species.
  • Measured caffeine permeability (20.8 × 10⁻⁶ cm s⁻¹) within a 10-minute timeframe.

Conclusions:

  • The microfluidic DIB assay provides a more biologically relevant model for permeability studies.
  • This platform enables high-throughput drug permeability screening with small sample volumes and reduced assay times.