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06:50
Super-Resolution Live Cell Imaging of Subcellular Structures
Published on: January 13, 2021
TAG-1-assisted progenitor elongation streamlines nuclear migration to optimize subapical crowding
Mayumi Okamoto1, Takashi Namba, Tomoyasu Shinoda
1Department of Anatomy and Cell Biology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Nature Neuroscience
|September 24, 2013
Summary
Interkinetic nuclear migration (INM) prevents neural progenitor overcrowding. Disrupted INM leads to abnormal brain development, highlighting its crucial role in ensuring proper histogenesis.
Area of Science:
- Neuroscience
- Developmental Biology
- Cell Biology
Background:
- Neural progenitors undergo cell cycle-dependent interkinetic nuclear migration (INM) along the apicobasal axis.
- The mechanical contributions and regulation of INM by progenitor morphology are not fully understood.
Purpose of the Study:
- To investigate the mechanical role of INM in neural progenitors.
- To understand how progenitor morphology influences INM and brain development.
Main Methods:
- Knockdown of the cell-surface molecule TAG-1 in neocortical progenitors.
- In toto imaging and physical tests to analyze progenitor behavior and mechanical stress.
- Histological analysis to assess brain development.
Main Results:
- TAG-1 knockdown caused retraction of basal processes, leading to shortened progenitors.
- Shortened progenitors failed to undergo basalward INM, resulting in nuclear overcrowding.
- Overcrowded progenitors migrated into basal territories, disrupting normal brain histogenesis despite retaining stem-like properties.
Conclusions:
- INM is essential for preventing nuclear and somatic overcrowding in neural progenitors.
- Proper INM is critical for normal brain histogenesis.
- Progenitors may sense and respond to excessive mechanical stress, impacting developmental trajectories.
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