Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia
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Updated: May 7, 2026

Live Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers Using Genetically Encoded Calcium Indicators
Published on: January 19, 2024
Steven Su1, Siew Cheng Phua, Robert DeRose
11] Department of Biomedical Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, Maryland, USA. [2] Department of Cell Biology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA. [3] Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA. [4].
Abstract:
Visualization of signal transduction in live primary cilia constitutes a technical challenge owing to the organelle's submicrometer dimensions and close proximity to the cell body. Using a genetically encoded calcium indicator targeted to primary cilia, we visualized calcium signaling in cilia of mouse fibroblasts and kidney cells upon chemical or mechanical stimulation with high specificity, high sensitivity and wide dynamic range.
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