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Zebrafish Larvae as a Model to Evaluate Potential Radiosensitizers or Protectors
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Published on: August 25, 2022

Silver nanoparticles induce developmental stage-specific embryonic phenotypes in zebrafish.

Kerry J Lee1, Lauren M Browning, Prakash D Nallathamby

  • 1Department of Chemistry, Biochemistry, Old Dominion University, Norfolk, VA 23529, USA. xhxu@odu.edu.

Nanoscale
|September 24, 2013
PubMed
Summary

Silver nanoparticles (Ag NPs) diffuse into zebrafish embryos at all developmental stages. Ag NPs cause stage- and dose-dependent nanotoxicity, with early developmental stages being most sensitive, impacting cell differentiation.

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Area of Science:

  • Nanomaterials Science
  • Developmental Biology
  • Toxicology

Background:

  • Nanomaterials offer potential in biological applications, but biocompatibility and specificity are concerns.
  • Silver nanoparticles (Ag NPs) are widely studied, necessitating an understanding of their embryonic effects.

Purpose of the Study:

  • To investigate the transport, biocompatibility, and toxicity of Ag NPs in zebrafish embryos across different developmental stages.
  • To determine stage-specific sensitivities and dose-dependent effects of Ag NP exposure.

Main Methods:

  • Utilized single nanoparticle plasmonic spectroscopy to study Ag NP transport.
  • Exposed zebrafish embryos (cleavage to hatching stages) to varying concentrations of Ag NPs (0-0.7 nM) for 2 hours.
  • Monitored for stage- and dose-dependent phenotypic changes and nanotoxicity.

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Published on: August 25, 2022

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Main Results:

  • Ag NPs diffused into all five embryonic stages studied, with stage-independent diffusion.
  • Significant stage- and dose-dependent nanotoxicity observed; late-segmentation embryos were most sensitive (CNP,c << 0.02 nM).
  • Cleavage-stage embryos showed diverse abnormalities, while hatching-stage embryos were most resistant (94% normal development).

Conclusions:

  • Ag NP toxicity is stage-specific, targeting critical early developmental events and cell differentiation.
  • Zebrafish embryonic development reveals differential sensitivity to Ag NPs, not acting as simple poisons.
  • Findings suggest potential for targeted developmental studies and therapies using Ag NPs.