Related Experiment Video
Updated: May 7, 2026
Multiplexed Live-Cell Imaging for Drug Responses in Patient-Derived Organoid Models of Cancer
Published on: January 5, 2024
Rapid differentiation and quantification of live/dead cancer cells using differential photochemical behavior of
1Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, 80424, Taiwan.
Abstract:
This paper demonstrates for the first time a simple analytical method for differentiation and quantification of dead/live cancer cells using acridine orange (AO) enabled fluorescence spectroscopic techniques. Based on the differential fluorescence (live cells fluoresce green and dead cells orange) exhibited when intercalated with AO, the live/dead cells can be easily differentiated. The optimal AO concentration for enhanced sensitive differentiation has been optimized as 0.001%. These studies offer a promising application for rapid differentiation and quantification of live/dead cells in the case of cytotoxic treatment/therapies within several minutes.
