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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
Genetic Screens02:46

Genetic Screens

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Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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Related Experiment Video

Updated: May 7, 2026

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
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Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

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Next-generation mapping of genetic mutations using bulk population sequencing.

Ryan S Austin1, Steven P Chatfield, Darrell Desveaux

  • 1Southern Crop Protection and Food Research Centre, Agriculture & Agri-Food Canada, London, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|September 24, 2013
PubMed
Summary

Next-generation sequencing rapidly maps genetic mutations in Arabidopsis by analyzing pooled F2 populations. This method efficiently identifies causal mutations by pinpointing homozygous regions linked to the mutant phenotype.

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Last Updated: May 7, 2026

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
11:02

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Published on: October 18, 2013

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

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Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
11:15

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

Area of Science:

  • Genomics
  • Plant Science
  • Arabidopsis thaliana research

Background:

  • Next-generation sequencing (NGS) enables rapid genetic mutation mapping.
  • Whole-genome resequencing of pooled F2 populations is a powerful tool for genetic analysis.
  • Understanding genetic mutations is crucial for plant breeding and functional genomics.

Purpose of the Study:

  • To describe detailed procedures for mapping genetic mutations in Arabidopsis using NGS.
  • To outline methods for identifying causal mutations in recessive and dominant phenotypes.
  • To provide a framework for analyzing mutations caused by point mutations, insertions, or deletions.

Main Methods:

  • Whole-genome resequencing of pooled F2 mapping populations exhibiting a mutant phenotype.
  • Alignment of sequence data to a reference genome.
  • Identification and filtering of polymorphisms to locate homozygous, nonrecombinant regions harboring mutations.

Main Results:

  • Successful identification of homozygous regions linked to recessive mutations through genetic hitchhiking.
  • Development of a strategy to efficiently narrow down potential causal mutations.
  • Adaptability of the method for various mutation types, including dominant mutations and indels.

Conclusions:

  • NGS-based whole-genome resequencing of pooled populations is an effective strategy for mapping genetic mutations in Arabidopsis.
  • The described methods facilitate rapid identification of causal mutations, accelerating genetic research.
  • The approach is versatile and can be extended to complex genetic scenarios beyond simple recessive point mutations.