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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: May 7, 2026

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

Published on: October 4, 2012

Flow cytometry and sorting in Arabidopsis.

David W Galbraith¹

¹School of Plant Sciences, University of Arizona, Tuczon, AZ, USA.

Methods in Molecular Biology (Clifton, N.J.)

|September 24, 2013

Summary

Flow cytometry and cell sorting are powerful tools for analyzing plant cells, advancing bioscience and agriculture. This study details methods for using these techniques with Arabidopsis thaliana for deeper biological insights.

Area of Science:

Plant biology
Cell biology
Biotechnology

Background:

Flow cytometry and cell sorting are established techniques for cellular population analysis.
Applications in higher plants have grown significantly over the last 30 years, aiding bioscience and agriculture.
Plant cells present unique challenges for flow cytometry due to their complex tissue structures and larger size.

Purpose of the Study:

To detail the application of flow cytometry and cell sorting in the model plant species Arabidopsis thaliana.
To explore fluorescence in vivo labeling for specific cell type identification.
To investigate fluorescence-activated sorting of protoplasts and nuclei for downstream analyses like transcriptomics.

Main Methods:

Utilizing flow cytometry for analyzing cellular populations in higher plants.

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Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Published on: May 29, 2010

Fluorescence Activated Cell Sorting of Plant Protoplasts

Fluorescence Activated Cell Sorting of Plant Protoplasts

Published on: February 18, 2010

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Last Updated: May 7, 2026

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

Published on: October 4, 2012

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Published on: May 29, 2010

Fluorescence Activated Cell Sorting of Plant Protoplasts

Fluorescence Activated Cell Sorting of Plant Protoplasts

Published on: February 18, 2010

Employing fluorescence in vivo labeling techniques for targeted cell identification.

Performing fluorescence-activated cell sorting (FACS) on protoplasts and nuclei.

Conducting transcriptome analyses on sorted cellular components.

Main Results:

Demonstrated successful fluorescence in vivo labeling of specific cell types in Arabidopsis.
Achieved efficient sorting of protoplasts and nuclei using fluorescence-activated methods.
Generated transcriptome data from sorted plant cells, providing insights into cellular functions.

Conclusions:

Flow cytometry and cell sorting are highly effective for dissecting cellular complexity in plants like Arabidopsis thaliana.
These methods offer valuable approaches for fundamental research and agricultural applications.
Further development promises enhanced understanding of plant cell interactions and functions.