Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 7, 2026

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
06:53

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

High-resolution optical tweezers for single-molecule manipulation.

Xinming Zhang1, Lu Ma, Yongli Zhang

  • 1Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut.

The Yale Journal of Biology and Medicine
|September 24, 2013
PubMed
Summary

Optical tweezers precisely measure piconewton forces governing biomacromolecule dynamics. This review highlights their use in studying protein folding and DNA-translocating molecular motors, revealing complex multi-scale behaviors.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of the cell wall proteins associated with the softening of <i>Lycium barbarum</i> L. fruit by using iTRAQ technology.

Food chemistry. Molecular sciences·2022
Same author

Using 7.0 T cardiac magnetic resonance to investigate the effect of estradiol on biventricular structure and function of ovariectomized rats exposed to chronic hypobaric hypoxia at high altitude.

Archives of biochemistry and biophysics·2022
Same author

Problematic Social Media Use and Depressive Outcomes among College Students in China: Observational and Experimental Findings.

International journal of environmental research and public health·2022
Same author

Comparative Proteomic Analysis of Plasma Membrane Proteins in Rice Leaves Reveals a Vesicle Trafficking Network in Plant Immunity That Is Provoked by Blast Fungi.

Frontiers in plant science·2022
Same author

Multimodal electrochemistry coupled microcalorimetric and X-ray probing of the capacity fade mechanisms of Nickel rich NMC - progress and outlook.

Physical chemistry chemical physics : PCCP·2022
Same author

Corneal morphology correlates with choriocapillaris perfusion in myopic children.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie·2022

Area of Science:

  • Biophysics
  • Single-molecule biophysics
  • Molecular dynamics

Background:

  • Forces at the piconewton level are crucial for biomacromolecule structure and dynamics.
  • These forces regulate essential processes like protein folding, assembly, and molecular motor function.
  • Understanding these dynamics requires high-resolution measurement techniques.

Purpose of the Study:

  • To introduce the principles and applications of optical tweezers.
  • To review the use of optical tweezers in studying protein folding dynamics.
  • To discuss the characterization of DNA translocation by molecular motors using optical tweezers.

Main Methods:

  • Utilizes optical tweezers to probe forces, structures, and dynamics at the single-molecule level.
  • Analyzes folding pathways of coiled coil proteins (pIL and SNARE complex).
Keywords:
DNA translocationSNARE proteinsmolecular motorsoptical tweezersprotein foldingsingle-molecule manipulation

More Related Videos

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements
08:50

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements

Published on: May 12, 2023

Related Experiment Videos

Last Updated: May 7, 2026

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
06:53

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements
08:50

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements

Published on: May 12, 2023

  • Investigates DNA translocation properties of ATP-dependent chromatin remodeling complexes.

    • Main Results:

    • Revealed multiple folding intermediates and pathways for pIL and SNARE complexes.
    • Characterized detailed DNA translocation properties of chromatin remodeling molecular motors.
    • Demonstrated the capability of optical tweezers to quantitatively analyze complex multi-scale biomacromolecular dynamics.

    Conclusions:

    • Optical tweezers are a revolutionary tool for single-molecule biophysics.
    • The technique provides unprecedented resolution for studying biomacromolecule folding and motor function.
    • Future developments promise further insights into complex biological systems.