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Related Experiment Video

Updated: May 7, 2026

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Published on: December 1, 2015

Making milk: A new link between STAT5 and Akt1.

Carrie H Oliver¹, Christine J Watson

¹Department of Pathology; University of Cambridge; Cambridge, UK.

|September 24, 2013

Summary

The STAT5A pathway is crucial for mammary gland development and lactation. New research reveals the PI3K/Akt pathway induces prolactin, initiating lactation.

Area of Science:

Cell Biology
Developmental Biology
Endocrinology

Background:

The transcription factor STAT5A is vital for mammary gland development, controlling progenitor cell development and alveologenesis.
STAT5A regulates milk protein gene transcription and is activated by prolactin, a key hormone for lactation.
The PI3K/Akt pathway is essential for synthesizing milk components like lipids and lactose.

Purpose of the Study:

To investigate the direct link between the STAT5A and PI3K/Akt pathways in mammary gland development.
To elucidate the role of the PI3K/Akt pathway in initiating lactation.

Main Methods:

The study builds upon existing research on STAT5A and PI3K/Akt pathways in mammary gland development.
Analysis of gene expression and cellular processes related to lactation.

Keywords:

Akt1 STAT5 autocrine differentiation mammary gland milk prolactin

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Last Updated: May 7, 2026

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

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Published on: December 1, 2015

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Published on: February 27, 2020

Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic

Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic

Published on: September 4, 2012

Main Results:

The PI3K/Akt pathway directly induces autocrine prolactin production.
This autocrine prolactin production is essential for the initiation of lactation.

Conclusions:

The PI3K/Akt and STAT5A pathways are directly linked, with PI3K/Akt initiating lactation via prolactin induction.
This finding provides new insights into the hormonal regulation of lactation and mammary gland function.