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Related Experiment Video

Updated: May 7, 2026

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans
09:38

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans

Published on: November 29, 2013

A method for measuring fatty acid oxidation in C. elegans.

Ida Coordt Elle1, Steven Vestergaard Rødkær, Julius Fredens

  • 1Department of Biochemistry and Molecular Biology; University of Southern Denmark; Odense, Denmark.

Worm
|September 24, 2013
PubMed
Summary
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This study introduces a method to measure fatty acid oxidation in live C. elegans. This technique reveals how starvation and the DAF-2 pathway impact lipid metabolism, offering new insights into cellular homeostasis.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Caenorhabditis elegans is a key model organism for studying lipid metabolism.
  • Research has focused on lipid accumulation, with less attention on fatty acid degradation's role in homeostasis.
  • Fatty acid degradation is crucial for maintaining cellular lipid balance.

Purpose of the Study:

  • To establish a method for quantifying complete fatty acid oxidation in live C. elegans.
  • To investigate the impact of physiological conditions and genetic factors on fatty acid oxidation rates.
  • To provide a tool for dissecting the genetic regulation of beta-oxidation.

Main Methods:

  • Utilized tritium-labeled fatty acids to measure tritiated H2O production via scintillation counting in live C. elegans.
Keywords:
C. elegansFatty acid oxidationbacterial dietdaf-2nhr-49

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Related Experiment Videos

Last Updated: May 7, 2026

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans
09:38

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans

Published on: November 29, 2013

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans
08:10

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

Published on: February 23, 2019

Measuring Oxidative Stress Resistance of Caenorhabditis elegans in 96-well Microtiter Plates
08:10

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Published on: May 9, 2015

  • Employed sodium azide to inhibit the electron transport chain and boiling to confirm assay specificity.
  • Analyzed fatty acid oxidation rates in response to varying bacterial diets, starvation, and genetic mutations (NHR-49, DAF-2).

    • Main Results:

    • Developed a reliable method to measure fatty acid oxidation in C. elegans.
    • Confirmed that sodium azide and boiling significantly inhibit H2O production, validating the assay.
    • Observed differential oxidation rates based on bacterial food source.
    • Demonstrated increased fatty acid oxidation during starvation, independent of NHR-49.
    • Showed reduced fatty acid oxidation in DAF-2 mutants.

    Conclusions:

    • The developed methodology accurately quantifies fatty acid oxidation in C. elegans.
    • Fatty acid oxidation is modulated by diet, starvation, and the insulin signaling pathway (DAF-2).
    • This method is valuable for exploring gene and pathway functions in beta-oxidation regulation.