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RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

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Published on: August 21, 2019

MicroRNAs that interfere with RNAi.

Katlin B Massirer¹, Amy E Pasquinelli

¹Division of Biology; University of California San Diego; La Jolla, CA USA.

|September 24, 2013

Summary

This summary is machine-generated.

MicroRNAs (miRNAs) regulate RNA interference (RNAi) efficiency in C. elegans. Loss of miR-35-41 enhances exogenous RNAi and reduces endogenous RNAi, balancing small RNA pathways.

Keywords:

C. elegans RNAi lin-35 miR-35-41 miRNA retinoblastoma (Rb)

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Area of Science:

Molecular Biology
Genetics
Developmental Biology

Background:

MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are key regulators of gene expression.
RNA interference (RNAi) pathways utilize Dicer and Argonaute proteins to target messenger RNAs (mRNAs).
The interplay between miRNA and RNAi pathways is crucial for cellular processes.

Purpose of the Study:

To discuss the cross-regulation between miRNA and RNAi pathways.
To explore the implications of this cross-regulation on gene expression.
To highlight the role of miR-35-41 in modulating RNAi efficiency.

Main Methods:

The study discusses findings from Massirer et al. in C. elegans.
Analysis of the regulatory role of miR-35-41 on RNAi pathways.
Investigation of the connection between miR-35-41, lin-35/Rb, and RNAi sensitivity.

Main Results:

A family of miRNAs, miR-35-41, regulates RNAi efficiency in C. elegans.
Loss of miR-35-41 leads to enhanced exogenous RNAi (exo-RNAi) sensitivity.
Loss of miR-35-41 results in reduced endogenous RNAi (endo-RNAi) effectiveness.
miR-35-41 regulates RNAi through lin-35, the C. elegans homolog of the Retinoblastoma (Rb) gene.
LIN-35/Rb protein accumulation is dependent on miR-35-41.

Conclusions:

miRNAs, specifically miR-35-41, play a critical role in balancing RNAi pathways.
This cross-regulation between miRNAs and RNAi provides a new layer of control over gene expression.
The findings exemplify the intricate cross-regulation within small RNA-directed pathways.