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Related Concept Videos

Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
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Protein Complexes with Interchangeable Parts01:57

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Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
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Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
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Related Experiment Video

Updated: May 7, 2026

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
10:58

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

Published on: July 25, 2013

NextGen protein design.

Nicholas Sawyer1,2, Elizabeth B Speltz1, Lynne Regan1,2,3

  • 1Department of Molecular Biophysics and Biochemistry, Yale University, 266 Whitney Avenue, New Haven, CT 06511, U.S.A.

Biochemical Society Transactions
|September 25, 2013
PubMed
Summary

Protein engineering advances designed protein-protein interactions for applications, with some proteins in clinical trials. However, rational protein design faces challenges requiring significant redesign of unsuccessful attempts.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Designed protein-protein interactions represent a significant advancement in protein engineering.
  • Several designed proteins are currently undergoing clinical trials, highlighting the field's progress.

Purpose of the Study:

  • To review the successes, challenges, and limitations of current rational protein design strategies.
  • To provide an overview of the state-of-the-art in protein engineering for designed interactions.

Main Methods:

  • Literature review of recent advancements in rational protein design.
  • Analysis of case studies where designed proteins have been applied.
  • Discussion of common pitfalls and redesign requirements in protein engineering.

Main Results:

  • Successful applications of designed protein-protein interactions are emerging.
  • A notable number of designed proteins have reached clinical trial stages.
  • Redesign is frequently necessary due to designs not meeting expectations.

Conclusions:

  • Rational protein design has achieved notable successes but still faces considerable challenges.
  • Further refinement of design methodologies is needed to improve predictability and reduce redesign efforts.
  • The field holds significant promise for future therapeutic and biotechnological applications.