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Updated: May 7, 2026

Quantitative 31P NMR Analysis of Lignins and Tannins
Published on: August 2, 2021
Tannin structural elucidation and quantitative ³¹P NMR analysis. 1. Model compounds
Federica Melone1, Raffaele Saladino, Heiko Lange
1Department of Chemical Sciences and Technologies, University of Rome Tor Vergata , Via della Ricerca Scientifica, 00133 Rome, Italy.
Abstract:
Tannins and flavonoids are secondary metabolites of plants that display a wide array of biological activities. This peculiarity is related to the inhibition of extracellular enzymes that occurs through the complexation of peptides by tannins. Not only the nature of these interactions, but more fundamentally also the structure of these heterogeneous polyphenolic molecules are not completely clear. This first paper describes the development of a new analytical method for the structural characterization of tannins on the basis of tannin model compounds employing an in situ labeling of all labile H groups (aliphatic OH, phenolic OH, and carboxylic acids) with a phosphorus reagent. The ³¹P NMR analysis of ³¹P-labeled samples allowed the unprecedented quantitative and qualitative structural characterization of hydrolyzable tannins, proanthocyanidins, and catechin tannin model compounds, forming the foundations for the quantitative structural elucidation of a variety of actual tannin samples described in part 2 of this series.
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