Related Experiment Video
Updated: May 7, 2026

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
Published on: July 14, 2015
Wide-bore P-methylstyrene-co-dimethylbis(p-vinylbenzyl) silane Based-monoliths Columns for Separation of Peptides and
Abstract:
Wide-bore monolithic columns of p-methylstyrene-co-dimethylbis(p-vinylbenzyl)silane were prepared within glass columns (100 × 3 mm I.D.). Monolithic columns differing in the amount of initiator were fabricated. The pressure drop vs. flow-rate measurements and the relatively low swelling propensity factor indicated the good cross-linking homogeneity and good mechanically stability. The chromatographic performance of monoliths prepared was assessed by analysis of protein and peptide mixtures. Five proteins were separated in less than 2 minutes. Moreover, a mixture of 9 peptides was separated in less than 11 minutes, aside from the co-elution of vasopressin [arg8] and methionine enkephalin.
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Principles Of Column Chromatography
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Types Of Column Chromatography
Gel Filtration Chromatography
When the...
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
