Imatinib Quantification in Human Serum for Clinical Purposes using High-performance Liquid Chromatography with a...

Area of Science:

• Analytical Chemistry
• Pharmacology

Background:

• Imatinib is a crucial drug in modern medicine, necessitating efficient quantification methods.
• Existing quantification techniques can be expensive and time-consuming.
• There is a need for accessible and rapid analytical methods for imatinib in biological samples.

Purpose of the Study:

• To develop and validate a fast, economical, and effective High-Performance Liquid Chromatography (HPLC) method.
• To quantify imatinib concentration in human serum samples.
• To provide a practical method for therapeutic drug monitoring of imatinib.

Main Methods:

• Utilized a simple sample preparation involving Extrelut® NT 3 extraction tubes and common solvents.
• Employed a Marcherey-Nagel Lichrospher 100-5 RP8 column with a specific mobile phase (0.05 M H3PO4/KH2PO4-acetonitrile, 7:3 v/v).
• Analyzed 50 µL serum samples using HPLC with a diode array detector at 265 nm, with a run time of 15 minutes.

Main Results:

• Achieved a limit of quantification of 30 ng/mL for imatinib.
• Demonstrated linearity for imatinib quantification in the range of 0.1 to 10 µg/mL.
• The developed method is fast, cost-effective, and suitable for routine analysis.

Conclusions:

• A validated, rapid, and economical HPLC method for imatinib quantification in human serum has been successfully developed.
• This method simplifies sample preparation and utilizes readily available materials and equipment.
• The technique is suitable for therapeutic drug monitoring and clinical applications, addressing the need for accessible quantification.