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Crystallization and preliminary X-ray investigation of recombinant Lactobacillus leichmannii nucleoside
W J Cook1, S A Short, S E Ealick
1Department of Pathology, University of Alabama, Birmingham 35294.
The Journal of Biological Chemistry
|February 15, 1990
Abstract:
Crystals of recombinant bacterial nucleoside deoxyribosyltransferase have been grown from solutions of ammonium sulfate. The crystals are cubic, space group I23 or I2(1)3; the axial length is 151.1(2) A. The crystals are stable to x-rays for at least 5 days and diffract beyond 2.8-A resolution. It appears that the molecule, which is a hexamer, utilizes the symmetry of the space group, resulting in two or three subunits per asymmetric unit.