DIFFERENCES IN IONIC CURRENTS BETWEEN CANINE MYOCARDIAL AND PURKINJE CELLS

An electrophysiological analysis of canine single ventricular myocardial (VM) and Purkinje (P) cells was carried out by means of whole cell voltage clamp method. The following results in VM versus P cells were obtained. I_Na3 was present, had a threshold negative to the fast activating-inactivating I_Na1, its slow inactivation was cut off by I_Na1, and contributed to Na⁺ influx at I_Na1 threshold. I_Na1 was smaller and had a less negative threshold. There was no comparable slowly inactivating I_Na2, accounting for the shorter action potential. Slope conductance at resting potential was about double and decreased to a minimum value at the larger and less negative I_K1 peak. The negative slope region of I-V relation was smaller during fast ramps and larger during slow ramps than in P cells, occurred in the voltage range of I_K1 block by Mg²⁺, was not affected by a lower V_h and TTX and was eliminated by Ba²⁺, in contrast to P cells. I_Ca was larger, peaked at positive potentials and was eliminated by Ni²⁺. I_to was much smaller, began at more positive values, was abolished by less negative V_h and by 4-aminopyridine, included a sustained current that 4-aminopyridine decreased but did not eliminate. Steeper ramps increased I_K1 peak as well as the fall in outward current during repolarization, consistent with a time-dependent block and unblock of I_K1 by polyamines. During repolarization, the positive slope region was consistently present and was similar in amplitude to I_K1 peak, whereas it was small or altogether missing in P cells. The total outward current at positive potentials comprised a larger I_K1 component whereas it included a larger I_to and sustained current in P cells. These and other results provide a better understanding of the mechanisms underlying the action potential of VM and P cells under normal and some abnormal (arrhythmias) conditions.