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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: May 7, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

[Recent progress in multi-stage mass spectrometry-based quantitative proteomics].

Ying Zhang1, Pengyuan Yang, Haojie Lu

  • 1Department of Chemistry, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China.

Se Pu = Chinese Journal of Chromatography
|September 26, 2013
PubMed
Summary
This summary is machine-generated.

Quantitative proteomics utilizes mass spectrometry (MS) for peptide measurement. This review focuses on MS(n)-based quantitation, comparing its methods, benefits, and drawbacks for advancing proteome research.

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A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
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Last Updated: May 7, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
09:00

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Quantitative proteomics is a rapidly advancing field.
  • Mass spectrometry (MS)-based techniques are crucial for quantitative proteomics.
  • MS-based methods are categorized into MS(1) and MS(n) quantitation.

Purpose of the Study:

  • To review the progress of MS(n)-based quantitation approaches.
  • To compare the advantages and disadvantages of MS(n) methods.
  • To provide a future outlook for MS(n)-based quantitation.

Main Methods:

  • Literature review of MS(n)-based quantitative techniques.
  • Comparative analysis of different MS(n) methods.
  • Discussion of current trends and future prospects.

Main Results:

  • Summary of recent advancements in MS(n) quantitation.
  • Detailed comparison of the strengths and weaknesses of various MS(n) techniques.
  • Identification of key trends shaping the future of MS(n) quantitation.

Conclusions:

  • MS(n)-based quantitation is a key area in proteomics.
  • Understanding the pros and cons of MS(n) methods is vital for researchers.
  • The field is expected to see continued innovation and development.